FMOC-Arg(Pbf)-OH (CAS# 154445-77-9)
Umngcipheko kunye noKhuseleko
| Iimpawu ezinobungozi | Xi – Irritant |
| Iikhowudi zoMngcipheko | 36/37/38 – Ukucaphukisa amehlo, inkqubo yokuphefumula kunye nolusu. |
| Inkcazelo yoKhuseleko | S26 – Xa udibana namehlo, hlambulula ngokukhawuleza ngamanzi amaninzi kwaye ufune iingcebiso zonyango. S36 – Nxiba iimpahla ezifanelekileyo ezikhuselayo. |
| WGK eJamani | 1 |
| Ikhowudi ye-HS | 2935 90 90 |
| IKlasi yeeNgozi | OKUTHUMEKAYO |
Intshayelelo
Iqela lokukhusela i-FMOC liqela elisetyenziswa ngokuqhelekileyo le-amino acid elikhusela iqela elisebenzayo le-amino le-arginine. Oku kulandelayo kukwazisa kwezinye iipropati, ukusetyenziswa, iindlela zokulungiselela, kunye nolwazi lokhuseleko lwe-Fmoc-Protective Radical:
Umgangatho:
Iqela elikhuselayo le-FOC liqela elikhuselayo elikhutshwayo elikhusela amaqela e-amino amino. Inokusabela kunye neqela le-amino kwi-arginine ngokuphendula kwe-esterification ukwenza i-Fmoc-arginine ester, ukuze kufezekiswe injongo yokukhusela iqela le-amino. Kukho amaqela anevumba elimnandi kwimolekyuli yeqela elikhuselayo le-Fmoc elifunxa ngamandla ukukhanya kwe-UV, evumela ukususwa kweqela elikhuselayo le-Fmoc ukuba liqhutywe ngemisebe ye-UV okanye iindlela zekhemikhali.
Sebenzisa:
Amaqela akhuselayo e-FOC asetyenziswa ngokubanzi kwi-peptide synthesis kunye ne-slid-phase synthesis. Inokukhusela ngokufanelekileyo iqela le-arginine amino ukukhusela ukuphendula kwayo ecaleni ngexesha lokudibanisa. Kwi-peptide synthesis, iqela lokukhusela i-Fmoc linokususwa ngeemeko ze-alkaline, ukuvumela ukuhlanganiswa kweepolypeptides ukuba kuqhubeke.
Indlela:
Iqela lokukhusela i-Fmoc linokulungiswa ngokusabela kwe-Fmoc-Cl kunye ne-arginine. I-Fmoc-Cl yi-reagent ene-acidic kakhulu esebenza kunye neqela le-amino kwi-arginine ukwenza i-Fmoc-arginine ester. Ukusabela ngokuqhelekileyo kuqhutyelwa kwi-ethanol kwiqondo lokushisa legumbi ukuya kwiqondo lokushisa lokuhlambela lomkhenkce.
Ulwazi ngoKhuseleko:
IiRadicals zoKhuselo ze-FMOC zikhuselekile ukuba zisetyenziswe phantsi kweemeko eziqhelekileyo zelabhoratri, kodwa oku kulandelayo kufuneka kuqatshelwe:
- I-FMOC-CL i-agent ecasulayo kunye netyhefu, kufuneka kuthathelwe ingqalelo ukuphepha ukudibanisa nesikhumba, i-inhalation okanye ukungenisa. Sebenza kwindawo engena umoya kwaye unxibe izixhobo ezifanelekileyo zokuzikhusela.
- I-FMOC-Isiseko soKhuseleko sinepropati yokutshatyalaliswa okunamandla kwemisebe ye-ultraviolet, ngoko kufuneka kuthathelwe ingqalelo ukukhusela ukudibanisa ngokuthe ngqo kwesikhumba kunye namehlo ngexesha lokusetyenziswa, kwaye ugcine kude nemithombo yokukhanya eqinile.
- Ukhuseleko oluqinileyo lwe-asidi ye-hydrolysis efana ne-pentafluorophenylcarboxylic acid (TFA) idla ngokusetyenziswa ngexesha lokususwa kwamaqela akhuselayo e-Fmoc, kwaye kuyafuneka ukuba uqaphele ukuba umphunga we-TFA unokubangela umonakalo, ngoko ke kuyimfuneko ukuba usebenze kwindawo efanelekileyo. indawo engena umoya.
