fmoc-O-tert-butyl-D-tyrosine (CAS# 118488-18-9)
I-Fmoc-O-tert-butyl-D-tyrosine isetyenziswa ngokuqhelekileyo i-amino acid ekhuselweyo. Oku kulandelayo yinkcazo yobume bayo, ukusetyenziswa, ukuqulunqwa kunye nolwazi lokhuseleko:
Indalo:
I-Fmoc-O-tert-butyl-D-tyrosine yikristale eqinileyo emhlophe. I-organic compound kunye ne-chemical formula C30H31NO7 kunye ne-molecular weight of 521.57g / mol. Ikhompawundi iphuma kwi-tyrosine apho iqela le-amino lithwala i-Fmoc (i-9-fluorofluorenylformyl) iqela elikhuselayo kunye neqela le-carboxylic acid esterified nge-O-tert-butyl.
Sebenzisa:
I-Fmoc-O-tert-butyl-D-tyrosine isetyenziswa ngokuqhelekileyo njenge-amino acid ekhuselweyo kwi-peptide synthesis. Ngokuncamathisela iqela elikhuselayo le-Fmoc kwiqela le-amino, iimpendulo ezingafunekiyo zecala zinokuthintelwa ngexesha lokudibanisa. Isetyenziswa ngokubanzi kwi-slide phase synthesis kwaye ingasetyenziselwa ukudibanisa i-polypeptides kunye neeprotheni.
Indlela yokulungiselela:
Ukulungiswa kwe-Fmoc-O-tert-butyl-D-tyrosine ngokuqhelekileyo kuqhutyelwa yi-chemical synthesis. Okokuqala, i-tyrosine isabela nge-Fmoc-Cl (i-9-fluorofluorenylcarbonyl chloride) ukuvelisa i-Fmoc-O-tyrosine. I-Cesium tert-butyl bromide yongezwa kwi-reaction ukuze i-esterify iqela le-carboxylic acid ukwenza i-Fmoc-O-tert-butyl-D-tyrosine. Ekugqibeleni, imveliso ecocekileyo ifunyenwe ngamanyathelo e-crystallization, ukuhlamba kunye nokomisa.
Ulwazi ngoKhuseleko:
I-Fmoc-O-tert-butyl-D-tyrosine yikhompawundi ezinzile phantsi kweemeko eziqhelekileyo kwaye ayinakho ukuguquguquka okucacileyo kwiqondo lokushisa. Ngexesha lokusetyenziswa, kuyimfuneko ukuthobela iinkqubo zokhuseleko lwebhubhoratri, unxibe izixhobo ezifanelekileyo zokukhusela, kwaye ugweme ukudibanisa nolusu kunye namehlo. Xa uphatha okanye ugcina, kufuneka ugcinwe kwindawo eyomileyo, epholileyo kwaye kude nomlilo kunye ne-oxidizing agents. Ngexesha elifanayo, ukudibanisa kunye ne-oxidants eqinile kunye ne-acids eqinile kufuneka kugwenywe ukukhusela ukuphendula okuyingozi. Funa uncedo lwezonyango olukhawulezileyo ukuba ukungeniswa okanye ukuvezwa ngengozi kwikhompawundi.
