fmoc-L-4-hydroxyproline (CAS# 88050-17-3)

Intshayelelo

I-Fmoc-L-hydroxyproline (i-Fmoc-Hyp-OH) yi-amino acid ephuma kwi-amino acid eneempawu ezilandelayo kunye nokusetyenziswa:

Umgangatho:

- Imbonakalo: Umgubo omhlophe okanye omhlophe wekristale

-Ukunyibilika: Inyibilika kwizinyibilikisi zendalo ezifana neDMF, DMSO kunye nemethanol

- ixabiso lepKa: 2.76

Sebenzisa:

I-Fmoc-Hyp-OH isetyenziswa ikakhulu kwi-peptide synthesis kunye ne-peptide synthesis kwi-slide-phase synthesis

- Isebenza njengenxalenye yeqela elikhuselayo lokukhusela amaqela asebenzayo ekhonkco esecaleni le-amino acids ngexesha le-slide-phase synthesis ukuphepha ukuphendula okungalindelekanga kunye nokugcina ukukhetha.

Indlela:

I-Fmoc-Hyp-OH inokulungiswa ngokuphendula i-Fmoc-amino acids kunye ne-L-hydroxyproline kwi-solvent efanelekileyo. Iimeko zokusabela ngokuqhelekileyo ziquka ubushushu obufanelekileyo bokusabela kunye nesiseko esifanelekileyo se-catalyst, njenge-N,N-dimethylpyrrolidone (DMAP). Imveliso ephumayo ihlanjululwa ngamanyathelo afana nemvula, ukuhlamba kunye nokomisa.

Ulwazi ngoKhuseleko:

- I-FMOC-HYP-OH yi-compound ye-organic kwaye kufuneka iphathwe ngokuhambelana nemigaqo yokhuseleko lwebhubhoratri.

- Uthuli lunokuphefumlelwa kwaye ludibane nolusu, ngoko kufuneka kuthathelwe ingqalelo ukuphepha ukuphefumla ngokuthe ngqo okanye ukudibana.

- Ngexesha lenkqubo, izixhobo ezifanelekileyo zokuzikhusela ezifana neeglavu zaselabhoratri, ukhuseleko lwamehlo, iimpahla ezikhuselayo, njl.njl kufuneka zinxitywe.

- Kufuneka igcinwe ivalwe ngokuqinileyo kwindawo eyomileyo, epholileyo, kude nomlilo kunye nezinto ezinokutsha.