Fmoc-L-Aspartic acid-1-benzyl ester (CAS# 86060-83-5)

Intshayelelo

I-Fmoc-Asp-OBzl(i-Fmoc-Asp-OBzl) yikhompawundi esetyenziswa ikakhulu kwi-peptide synthesis kunye ne-slide phase synthesis.

Indalo:

I-Fmoc-Asp-OBzl yikristale emhlophe eqinileyo ene-solubility efanelekileyo kunye nokuzinza. Ifomula yayo yeekhemikhali yi-C33H29NO7 kunye nobunzima bayo be-molecular yi-555.6. Ineqela elikhuselayo le-fluorenyl (Fmoc) kunye neqela elikhuselayo le-benzoyl (Bzl) ukukhusela i-aspartic acid.

Sebenzisa:

I-Fmoc-Asp-OBzl njengeqela elikhuselayo linokusetyenziswa kwi-synthesis ye-peptides kunye neeprotheni. Ingasetyenziswa kubuchule bokwenziwa kwesigaba esiluqilima kunye nenyathelo lokususa iqela elikhuselayo kwimpendulo yepeptide synthesis. Kwi-synthesis, intsalela ye-aspartic acid inokuthi i-Fmoc-Asp-OBzl ikhuselwe kwisiqwenga se-peptide esinesiphumo sokuthintela ukusabela okungafunekiyo.

Indlela yokulungiselela:

Ukulungiswa kwe-Fmoc-Asp-OBzl ngokuqhelekileyo kufezekiswa ngokuhlanganiswa kweekhemikhali. Ngokukodwa, i-Fmoc-Asp-OBzl inokufumaneka ngokuphendula i-fluorenecyl chloride (Fmoc-Cl) nge-aspartic acid -1-benzyl ester (Asp-OBzl).

Ulwazi ngoKhuseleko:

I-Fmoc-Asp-OBzl yikhemikhali ekufuneka iphathwe elabhoratri. Landela iinkqubo ezifanelekileyo zokhuseleko lwaselabhoratri ngexesha lokuphatha, njengokunxiba izixhobo ezifanelekileyo zokukhusela (ezifana neeglavu, iiglasi, kunye needyasi zaselabhoratri) ukunqanda ukudibana nolusu, amehlo kunye nomgudu wokuphefumla. Ukongezelela, kufuneka igcinwe kwindawo eyomileyo, epholileyo, kunye nomlilo kunye nezinto ezinokutsha. Xa usebenzisa i-Fmoc-Asp-OBzl, ingqalelo kufuneka ihlawulwe kwityhefu kunye nokucaphuka kwayo, kwaye iqinisekise ukuba iqhutywe kwindawo ekhuselekileyo.