Isopropyl-beta-D-thiogalactopyranoside (CAS#367-93-1)
Umngcipheko kunye noKhuseleko
| Iikhowudi zoMngcipheko | I-R19 – Isenokwenza iiperoxide ezidubulayo R40 – Ubungqina obuncinane besiphumo somhlaza I-R66 – Ukutyhileka okuphindaphindiweyo kunokubangela ukomisa kolusu okanye ukuqhekeka R36/37/38 – Ukucaphuka kwamehlo, inkqubo yokuphefumla kunye nolusu. |
| Inkcazelo yoKhuseleko | S23 – Musa ukuphefumla umphunga. S24/25-Kuphephe ukudibana nolusu namehlo. S36/37 – Nxiba iimpahla ezifanelekileyo zokukhusela kunye neeglavu. S22 – Musa ukuphefumla uthuli. S36/37/39 – Nxiba iimpahla ezifanelekileyo zokukhusela, iiglavu kunye nokukhusela amehlo/ubuso. S27 – Khulula ngoko nangoko zonke iimpahla ezosulelekileyo. S26 – Xa udibana namehlo, hlambulula ngokukhawuleza ngamanzi amaninzi kwaye ufune iingcebiso zonyango. |
| WGK eJamani | 3 |
| FLUKA BRAND F IIKHOWUDI | 10 |
| I-TSCA | Ewe |
| Ikhowudi ye-HS | 29389090 |
Intshayelelo
I-IPTG yinto ebangela umsebenzi we-β-galactosidase. Ngokusekelwe kolu phawu, xa iVector DNA yochungechunge lwe-pUC (okanye enye i-DNA ye-vector ene-lacZ gene) iguqulwa ngeeseli zokucinywa kwe-lacZ njengenginginya, okanye xa i-DNA ye-M13 ye-vector idluliselwe, ukuba i-X-gal kunye ne-IPTG zongezwa. ukuya kwiplate medium, ngenxa ye-α-complementarity ye-β-galactosidase, i-gene recombinant inokukhethwa ngokulula ngokweekholoni ezimhlophe. (okanye amacwecwe) avela. Ukongeza, inokuphinda isetyenziswe njenge-inducer yokubonisa iivektha zokubonisa kunye nabakhuthazi abanje nge-lac okanye i-tac. Inyibilika emanzini, i-methanol, i-ethanol, i-acetone, i-chloroform, i-soluble kwi-ether. I-inducer ye-β-galactosidase kunye ne-β-galactosidase. Ayinahydrolyzed nge-β-galactoside. Sisisombululo se-substrate ye-thiogalactosyltransferase. Yenziwe: I-IPTG inyibilika emanzini, kwaye emva koko inzala ukulungiselela isisombululo sokugcina (0 · 1M). Ukugxininiswa kwe-IPTG yokugqibela kwi-plate yesalathisi kufuneka ibe yi-0 · 2mM.
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