N(alpha)-fmoc-N(epsilon)-(2-chloro-Z)-L-lysine(CAS# 133970-31-7)

I-Fmoc-(2-chlorobenzyloxycarbonyl) i-lysine yinto eqhelekileyo ephuma kwi-amino acid enezi mpawu zilandelayo:1. Ukubonakala: i-crystalline powder emhlophe;
2. ifomula yemolekyuli: C26H24ClNO5;
3. Ubunzima bemolekyuli: 459.92g / mol;
4. I-Solubility: I-soluble in organic solvents ezifana ne-dimethyl sulfoxide (DMSO), i-dimethyl formamide (DMF), i-dichloromethane, njl., i-insoluble emanzini;
5. Indawo yokunyibilika: malunga ne-170-175 ° C. Ukusetyenziswa okuphambili kwe-Fmoc-(2-chlorobenzyloxycarbonyl) i-lysine njengeqela elikhuselayo kunye nelokusebenza kwi-synthesis ye-polypeptides. Iqela layo le-carboxyl linokusetyenziswa ukwenza i-ester, ethi emva koko ingene kwi-condensation reaction kunye nentsalela ye-amino acid ukuze yenze ikhonkco le-polypeptide. Iqela le-Fmoc linokususwa ngokulula emva kokuba impendulo igqityiwe ukuveza i-amino moiety ekhuselweyo.

Indlela yokulungiselela i-Fmoc-(2-chlorobenzyloxycarbonyl) lysine ngokubanzi ibandakanya la manyathelo alandelayo:

1. ukuphendula i-lysine kunye ne-N-hydroxybutyrimide (Pbf) ukwazisa iqela elikhuselayo;
2. ukuphendula i-lysine-Pbf derivative kunye ne-2-chlorobenzyl utywala ukwenza i-Fmoc-(2-chlorobenzyloxycarbonyl) i-lysine;
3. Imveliso ikhutshwe nge-solvent efanelekileyo kwaye ihlanjululwe nge-crystallization ukufumana imveliso ecocekileyo.

Ngokumalunga nolwazi lokhuseleko, i-Fmoc-(2-chlorobenzyloxycarbonyl) lysine sisixhobo sokusebenza ngemichiza kwaye amanyathelo afanelekileyo okhuseleko kufuneka athathwe. Izixhobo ezifanelekileyo zokukhusela, ezinjengeeglavu zaselebhu, iiglasi, kunye nempahla yaselebhu, kufuneka zinxitywe ngexesha lovavanyo. Kuphephe ukuphefumla umgubo okanye izisombululo, kuphephe ukudibana nolusu kunye namehlo. Kwimeko yoqhagamshelwano ngengozi, hlambulula ngokukhawuleza ngamanzi amaninzi kwaye ufune uncedo lwezonyango. Qinisekisa ukuba isetyenziswa kwindawo ekhuselekileyo zelebhu kwaye igcinwe ngokufanelekileyo ukuthintela iingozi.