N-(9-Fluorenylmethyloxycarbonyl)-N'-trityl-D-asparagine (CAS# 180570-71-2)
Umngcipheko kunye noKhuseleko
| WGK eJamani | 3 |
| Ikhowudi ye-HS | 29242990 |
| IKlasi yeeNgozi | OKUTHUMEKAYO |
N-(9-Fluorenylmethyloxycarbonyl)-N'-trityl-D-asparagine (CAS# 180570-71-2) Intshayelelo
2. Sebenzisa: I-Fmoc-D-Asn (Trt) -OH i-reagent ebalulekileyo esetyenziswe kwintsimi ye-polymer synthesis kunye ne-biochemistry. Ngokuqhelekileyo isetyenziselwa ukukhusela izicwangciso zeqela kwi-synthesis yesigaba esiqinileyo ukukhusela amaqela e-amino kwii-amino acids okanye amaqhekeza e-peptide. Eli qela elikhuselayo linokususwa nge-hydrofluoric acid phantsi kweemeko ze-ammonia-alkaline emva kokuhlanganiswa.
3. Indlela yokulungiselela: Indlela ye-Fmoc-D-Asn (Trt) -OH inzima kakhulu, ngokuqhelekileyo kufuneka isebenzise i-multi-step reaction. Indlela yokwenziwa eqhelekileyo kukusabela i-trityl amine kunye ne-N-ekhuselweyo ye-D-asparagine, kwaye emva koko yenza i-deprotection reaction phantsi kweemeko ezifanelekileyo zokufumana imveliso yokugqibela.
4. Ulwazi lokhuseleko: Nangona i-Fmoc-D-Asn(Trt)-OH ikhuselekile ngokufanelekileyo phantsi kweemeko zovavanyo jikelele, inokubangela ukucaphuka kwamehlo, ulusu kunye nomgudu wokuphefumula. Ukusetyenziswa kufuneka kulandele iinkqubo zaselabhoratri kwaye kusetyenziswe imilinganiselo yokukhusela efanelekileyo efana neeglavu, iiglasi kunye nempahla yokukhusela. Ngexesha lokusetyenziswa kunye nokugcinwa, gcina kude nomlilo kunye ne-oxidizing agents, kwaye ugcine kwindawo eyomileyo, epholileyo.
